To determine which HLA antigens are associate with rheumatoid arthritis (RA), the HLA class strand comformation polymorphism (SSCP) in 105 rheumatoid arthritis (RA) and 235 healthy Koreans.
@ES The results were as follows:
@EN 1. The genotype frequency of HLA-DRBI*04 was 56.2% in RA patients and 29.8% in healthy normal controls (RR=3.0, p<10E-5), and the frequencies of the HLA-DRB1*15 (RR=0.5, p<0.05), and*14 (RR=0.4, p<0.05) were decrease in RA patients compared
with
healthy normal controls.
2. The frequencies of the HLA-DOA1*03 (RR=3.6, p<10E-5), DQB1*04 (RR=3.3, p<10E-3) alleles were increased and of the DQA1*01 (RR=0.4, p<10E-7), *o5(RR=0.5, p<0.05), DQB1*02 (RR=3.3, p<0.005), *06 (RR=0.6, p<0.01) alleles were decreased in RA
patients
compared with healthy normal controls.
3. The alleles of the HLA-DR4 subtypes (0401, 0403, 0404, 0405, 0406, 0407, 0410) were identified in Koreans, and the high frequent alleles were 0405 (11.1%), 0406 (6.4%), 0403 (5.1%) in healthy normal controls and 0405 (42.9%), 0406 (6.7%) in RA
patients. The frequency of the HLA-DRB1*0405 (RR=6.0, p<10E-6) was especially increased in RA patients compared with healthy normal controls.
4. HLA-DRB1*0405-DQA1*03 (HF=0.1876, p<10E-5) and HLA-DRB1*04 (HF=0.1160, p<10E7) haplotypes have significant positive linkage disequilibria. It suggests that HLA-DQA1*03 and DQB1*04 may react synergistically in the development of the RA along
with
HLA-DRB1*0405 alleles. These results showed that HLA-DRB1*0405 was strongly associated with rheumatoid arthritis in Koreans.
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